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Domestic self-developed nanopore gene sequencer moves towards matrix development, and qicarbon technology reissues new sequencers

On June 28,”Qi Zhili to talk about pathogen Qi carbon technology new product launch and online academic seminar” was successfully held. Qi carbon technology released the nanopore gene sequencer qnome-3841hex and related kits, providing a new solution for flexible sequencing scenarios.

Qicarbon technology online conference

As a new member of the homogeneous carbon qnome family, the successful release of qnome-3841hex marks the beginning of the matrix development of the domestic nanopore gene sequencer, which will meet the more diverse sequencing needs of the market and provide core support for research and application in various fields.

Six chips operate independently to respond to the market demand for flexible sequencing

At the press conference, xiedan, co-founder of qicarbon technology, said,”the nanopore gene sequencer qnome-3841hex is a desktop sequencer and an upgraded version of qnome-3841. Its biggest feature is that it has more flexible flux, supports up to 6 sequencing tasks to run independently, and can freely combine sequencing chips in the sequencing process, which is flexible and controllable, without sampling, and reduces startup costs to the greatest extent.”.

Nanopore gene sequencer qnome-3841hex

In terms of data output, qnome-3841hex is equipped with a newly upgraded sequencing chip qcell-384. A single chip can output 3G data. Under the environment of six chips running at the same time, one-time sequencing can obtain 18G data, meeting the sequencing needs of higher throughput.

In terms of accuracy and reading length, qnome-3841hex continues the characteristics of the previous generation of products. The single time accuracy remains at 90%and the consistency accuracy reaches 99.9%. Long reading length has always been a significant feature of nanopore single molecule gene sequencing. Theoretically, there is no restriction on the reading length of sequencing, which only depends on the reading length of the sample nucleic acid. Therefore, the reading length of qnome-3841hex ranges from 200bp to more than 2mbp.

In terms of application performance, qicarbon internal has carried out performance tests of different chip combinations on qnome-3841hex according to the needs of various scenarios, and conducted application tests in pathogen identification, microbial assembly, tumor fusion gene identification, forensic identification, low-depth sequencing of human genome and other directions, all of which have obtained unexpected results or indicators, indicating the universality and reliability of the application of qnome-3841hex.

As a desktop nanopore gene sequencer, qnome-3841hex is simple in appearance and easy to operate. It can get rid of the limitations of the central laboratory. In addition, its library is easy to prepare, analyzes while sequencing, and has the characteristics of real-time basecall. It helps to go to the front line at any time in public health emergencies, quickly find the source of the problem, and assist in rapid detection and comprehensively grasp the pathogen gene information in the pathogen Research scenario.

As for the new products released twice within half a year by Qitan, which is concerned by the industry, Xie Dan said,”As the homogeneous carbon qnome sequencing platform provides value in more and more scenarios and fields, the hard power of domestic nanopore gene sequencing technology has been witnessed and recognized. At the same time, the market side and application side have also given it more expectations:more flexible sequencing, higher throughput, lower cost, etc. after six months, homogeneous carbon technology and qnome-3841hex have delivered a new answer to the market.”

As an early experimenter of the homogeneous carbon nanopore gene sequencer, Professor liruichao of the school of veterinary medicine of Yangzhou University said that we feel that the homogeneous carbon qnome platform has obvious advantages in sequencing length. As an upgraded version, qnome-3841hex has significantly improved its flux, and is bound to enter more application scenarios. It is expected that the new product will boost the production of more scientific research achievements. The development of sequencing technology will greatly promote the progress of life science and medical research. It is expected that the domestic nanopore gene sequencing technology will be more mature and stable, which will benefit technical research and clinical application.

At the press conference, qicarbon technology also brought a PCR barcode library building kit to meet the needs of mixed sequencing of multiple samples on a single chip. The kit includes two modules:bar code kit and PCR library building kit. It provides 12 bar codes and supports the construction requirements of 6 groups of mixed libraries. The library building can be completed in 3 hours. It is simple and fast. With the full carbon qnome sequencing platform, it can greatly reduce the sequencing cost of a single sample.

Domestic nanopore gene sequencing technology enables microbial research to provide core support for rapid detection

Since the beginning of this year, the carbon nanopore gene sequencer and its supporting chips, reagents and other products have been successively delivered to the user, representing the official market entry of the domestic nanopore gene sequencer.

Taking the opportunity of the press conference, qicarbon technology also invited Professor Wang Hui, director of the laboratory department of people’s Hospital of Peking University, and researcher Yang hang of Wuhan Institute of Virology, Chinese Academy of Sciences to share the theme of the actual measurement and application exploration of nanopore gene sequencer in the field of microbial research.

Professor Wang Hui said that in the past half a year, he has cooperated with Qi carbon in bacterial genome assembly, metagenome sequencing and other projects, and aligned the carbon metagenome pathogen detection solution for clinical verification. We are very glad to see the breakthrough of homogeneous carbon nanopore gene sequencing technology. The homogeneous carbon qnome sequencing platform can quickly and stably detect pathogens, and the detection results of clinical positive samples are highly consistent with those of classical methods.

In addition, nanopore gene sequencing has unlimited potential in the field of drug-resistant bacteria detection. Researcher Yang hang said that in the research on bacteriophages, drug-resistant bacteria and their interactions, rapid and accurate gene sequencing technology is of great significance to scientific research and clinical practice. He hoped to continue to cooperate with the homogeneous carbon qnome sequencing platform and continue to provide value for the research on bacteriophage, bacterial interaction and bacteriophage tolerance.

As the core tool of life science and technology, the carbon homogeneous nanopore gene sequencer has also been applied in judicial criminal investigation, public health and epidemic prevention, animal and plant research, infection diagnosis and other fields. At present, more than 100 institutions have used the carbon homogeneous qnome sequencing platform. The domestic nanopore gene sequencing technology is enabling more and more scholars and researchers to make breakthroughs in research.

At the press conference, experts from Kunming Institute of zoology, Chinese Academy of Sciences, Beijing Tsinghua Changgeng Hospital Affiliated to Tsinghua University, and West China School of basic medicine and forensic medicine, Sichuan University pointed out that the homogeneous carbon qnome sequencing platform showed the excellent performance of domestic independently developed precision instruments, and performed well in sequencing stability and speed. They are looking forward to the continuous upgrading and optimization of homogeneous carbon nanopore single molecule sequencing technology to provide value in more fields.    

Take advantage of the trend to lead the domestic new generation of gene sequencing technology products towards matrix development

At present, the revolution of life science and technology has swept the world, accelerated its integration into economic and social development, and provided richer solutions for the rapid growth of human life and health needs. Biotechnology represented by gene sequencing has ushered in unprecedented development opportunities.

Recently, the national development and Reform Commission issued the”14th five year plan” for bioeconomy development, which is China’s first Five-Year Plan for bioeconomy. The plan proposes to accelerate the development of high-throughput gene sequencing technology, promote the innovation of a new generation of sequencing technology marked by single molecule sequencing, and continuously improve gene sequencing efficiency and reduce sequencing costs.

It can be said that the innovation of new generation sequencing technology has risen to the national strategic level. As Xie Dan said at the press conference,”nanopore gene sequencing technology has shown great potential and subversive power. We believe that the next few years will become a key window for the outbreak of nanopore gene testing technology and Application”.

Qicarbon technology will take advantage of the policy to continuously improve its scientific and technological innovation ability, drive product iteration, uphold an open and cooperative attitude, carry out scientific research cooperation in different research fields, further break the transformation barriers, promote the innovative application of the new generation of gene sequencing technology in various fields, and turn scientific and technological achievements into real productivity as soon as possible.