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Imaging technology has always been an important driving force to promote the development of biomedical fieldAs the main means of observing the micro world, micro imaging technology has advanced by leaps and bounds in recent years. Biomicroscopy has important applications in basic research of molecular mechanism, drug target discovery and disease diagnosis. The progress of fluorescence microscopy, confocal microscopy, electron microscopy, light sheet microscopy and other biological microscopy technologies has greatly promoted the development of life science.

Nine experts from Peking University, Xi’an Jiaotong University, Shanghai Fudan University, Chinese Academy of Sciences and head instrument manufacturers will give us a comprehensive introduction to the new applications and innovative progress of microscopy in the field of life sciences, from super-resolution microscopy to high-speed atomic force microscopy, from optical microscopy to light microscopy electron microscopyCorrelation imaging technology. We sincerely invite you to attend the domestic frontier micro feast!

Meeting time:9:00-17:30, August 10

Meeting schedule:

time

Report title

Guest speaker

9:00

Development, application and Prospect of super-resolution microscopic imaging technology

Li Dong (Institute of Biophysics, Chinese Academy of Sciences)

9:30

High space-time resolution structured light illumination microscopic imaging and optical micromanipulation

Lei Ming (Xi’an Jiaotong University)

10:00

undetermined

Leica microsystems

10:30

Computational super-resolution:using sparse deconvolution to overcome the physical resolution limit

Chen Liangyi (Peking University)

11:00

High resolution modular microscopic imaging system

highXiang (Yangtze River Delta Institute of optoelectronics, Peking University)

13:30

Two photonStimulated emission loss(STED)Integration and application of composite micro imaging technology

Zhang Yunhai (Suzhou Institute of biomedical engineering technology, Chinese Academy of Sciences)

14:00

细胞原位high分辨分子成像——Cryo-Electron TomographyProgress of sample preparation technology

Wang Xiangli(Thermo Fisher Scientific)

14:30

Light microscope electron microscope correlation imaging technology and Practice

Kong Yu (Center for excellence and innovation in brain science and intelligent technology, Chinese Academy of Sciences)

15:00

high光稳定性、high亮度荧光探针用于STED超分辨成像

Wang Lu (Fudan University)

Summary of some reports:

《Development, application and Prospect of super-resolution microscopic imaging technologyRegistration space

[Abstract] 本报告将介绍李栋课题组开展的high时空分辨成像技术研制工作。在In terms of microscope hardware, we have developed a multimodal structured light super-resolution microscope and a lattice light sheet super-resolution microscope system,实现了在活细胞条件下对多种生物过程进行high速、多色、长时程超分辨成像;在In terms of super-resolution image reconstruction software, algorithms such as feature extraction of Fourier domain attention mechanism are proposedBased on this, a Fourier domain attention convolution neural network is developed, which can obtain an imaging effect comparable to that of traditional super-resolution microscopy under low signal-to-noise ratio imaging conditionsThe application scope of traditional super-resolution microscope is significantly expanded

《High space-time resolution structured light illumination microscopic imaging and optical micromanipulation》Registration space

[Abstract] 研究团队发明了包括high速彩色结构光照明显微等多种新型显微技术和空频域联合变换图像重构算法(JSFR-SIM),结构光The spatial resolution of the super-resolution system is better than 90nm, and the reconstruction speed reaches the order of milliseconds, which can be reconstructed in real time.

《High resolution modular microscopic imaging system》Registration space

[Abstract] 本系统通过模块化设计,融合了光学衍射层析、Two photon荧光、宽场荧光三种显微成像模态, which combines non labeled and fluorescent labeled microscopic imaging techniques,能够对样品进行长时程、无损伤的三维成像观测,空间分辨率接近衍射极限。主要用于生物细胞、组织的high分辨成像,以及第三代半导体晶圆的质检,在生物医学及半导体领域具有广阔的应用。

《Two photon-Stimulated emission loss(STED)Integration and application of composite micro imaging technology》Registration space

[Abstract]  本报告介绍了STED成像关键技术、Two photon-Stimulated emission loss(STED)复合显微成像技术集成及仪器研制情况,仪器集成了Two photon成像和STED成像等功能,介绍了Two photon-STED成像在难治性癫痫等神经疾病的应用研究情况。

《Light microscope electron microscope correlation imaging technology and Practice》Registration space

[Abstract] CLEM技术将不同的显微技术集成应用于同一个细胞对象上,可获取多重信息和high分辨率而受到研究者广泛关注。在电镜里找到并拍摄荧光信号所在的目标区域,并让两种图像信息准确关联,是技术成功的关键。本报告介绍了CLEM技术在培养细胞、组织切片样品中的应用,通过克服电镜制样过程中样品形变、细胞位移等技术难点,在具有Z轴信息的共聚焦显微镜成像和连续切片场发射扫描电镜成像之间进行多级配准,Realize photoelectric correlation from cell to tissue level

Welcome to participate in the live broadcast. You can also interact with expert teachers to getOn site Q & AOpportunity!

Click to register!Registration space