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Progress in rapid detection of new crown neutralizing antibody by academician team of West Lake University

The rapid detection of new coronavirus antibodies, especially neutralizing antibodies, is not only conducive to the evaluation of the safety and efficacy of new coronavirus vaccines, but also helps to clarify many key issues of novel coronavirus immunology, epidemiology and personalized medicine.

The team of academician Mohamad Sawan of West Lake University used fiber optical biofilm interferometry (fo-bli, FIG. 1a) to realize the rapid detection of sars-cov-2 binding antibody and neutralizing antibody (Fig. 1b, 1c). The results were published in biosensors and bioelectronics.

Figure 1 Optical fiber structure (a), optical fiberSchematic diagram of detection of surface new crown binding antibody (b) and new crown neutralizing antibody (c).

Biofilm interference technology is a technology to detect the surface reaction of sensor by detecting the displacement change of interference spectrum. It is widely used to characterize the intermolecular affinity. This project skillfully uses 3,3 ‘- diaminobenzidine (DAB for short, the reaction substrate of horseradish peroxidase HRP) to amplify the signal and realize the accurate quantitative analysis of neocrown antibody. The special feature of this subject is that it realizes the detection of high sensitivity of binding antibody (10 ng/ml) and 13 minutes of the whole process, as well as the detection of high sensitivity of neutralizing antibody (10 ng/ml) and 7.5 minutes of the whole process (Fig. 2).

Figure 2 The detection process of fo-bli biosensor for new crown binding antibody and neutralizing antibody.

Through the preclinical analysis of a variety of new crown antibodies and new crown aptamers, and the clinical study of serum samples in healthy people after vaccination, the results produced by this technology are in good agreement with those produced by commercial kits. Considering the strong flexibility of the detection scheme, it can be used for rapid screening of neutralizing antibodies such as delta and Omicron. This technique is not only suitable for the individualized analysis of a single serum sample, but also suitable for high-throughput sample analysis. At present, the technical achievements have been applied for two national invention patents.

Dr. Bian Sumin, assistant researcher of cenbrain team in the school of technology of West Lake University, is the first author of this paper, Shang Min, researcher of Run Run Run Shaw Hospital Affiliated to the Medical College of Zhejiang University, is the co first author, and academician Mohammad Sawan, founder of cenbrain, is the corresponding author. This work has been supported by the National Natural Science Foundation of China and the leading innovation and entrepreneurship team project of Zhejiang Province.

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