Posted in: information

With the same liquid quality, where does a good hand come from| This kit is a breakthrough in health research

Where does the liquid hand come from? Great hands come from Agilent’s excellent users. Agilent launches the first episode of the liquid hand series——Scientific research kit of bleck Technology (Wuhan) Co., Ltd. (hereinafter referred to as bleck)

AboutMetabonomicsThis game of chess… She said:

  ”At present, for master’s students in analytical chemistry, they can develop a set of metabolite small molecule detection method based on uhplc-ms, which actually meets the graduation requirements. However, the stability, universality and convenience of this method in the detection of large samples are difficult to be guaranteed. As we all know, after graduation, this method is like a martial arts secret script, facing the risk of loss, and we need to make further progress based on this method It is even more difficult to optimize and improve.”

“Our initial idea was to further refine the developed new method into a stable and reliable commercial testing service. No matter how many or thousands of samples are tested, no matter what type of samples, no matter which batch of samples, we can get consistent results. It is as convenient for scientific research users as using computers. We only use simple input (clear what to test), and we do standardized output (Quantitative test results), rather than letting each scientific research user build a computer from scratch (developing test methods).”

  “我们服务过很多科研用户,有研究阿尔兹海默症的,有研究颅内动脉瘤的,有研究乙肝治疗效果的,还有研究抗结核药物新靶点的。科学家们有不同的研究方向,而我们要做的就是提供Metabonomics通用化的检测服务,帮助他们在病理生理、药物研发、营养学、环境科学等诸多领域中,加速新Biomark、新功能、新机制的发现过程。”

Yu men, deputy general manager of bleck

The pathogenesis of diseases and the changes of related metabolites have always been a hot spot in the field of life science research.Metabonomics通过检测技术,研究和发现人类某些标志物或代谢物的表型和规律,提前实现疾病的预防甚至治疗。而精准、快速、方便的检测设备和方案,也因此成为了疾病研究和治疗,以及整个Metabonomics发展的关键。

Agilent has been practicing“In China, for China”By providing Agilent’s cutting-edge, stable and high-performance product platform, as well as professional knowledge and technical teams, we can help more local partner enterprises achieve innovation and develop highly competitive new products and solutions for different market segments and needs.

Bleck technology R & D team and Agilent’s ultra high performance liquid chromatography tandem mass spectrometry (UHPLC MS/MS) platform

Recently, it is based on Agilent’s超高效液相色谱-串联质谱(UHPLC-
MS/MS)
platform,Metabonomics科研服务公司——博莱克科技(武汉)有限公司,研发了一系列代谢物小分子定量检测方式,推出3套定量检测试剂盒产品,可针对实验动物和人体血样、尿样中的20种蛋白质氨基酸、12种神经递质和6种儿茶酚胺进行精准定量检测,让医疗健康和Metabonomics领域的科研和检测人员,都能便捷、快速地完成多种指标的检测。

Test indicators of kit

Usually, expensive detection equipment and complex detection methods are needed to accurately measure the absolute structure of metabolites in human blood, urine, saliva and other samples, and quantify their concentrations and changes. Without a kit, it may take several months to develop a stable detection method according to the type, number, method, equipment and personnel proficiency of the detection target. The metabolite quantitative detection kit has the advantages of reagent integration, method standardization, convenient operation and so on, which can greatly improve the experimental efficiency and laboratory management efficiency of researchers, avoid the lengthy and complicated development and testing time of detection methods, and let researchers pay more attention to the data analysis itself rather than the development of instrument methods.

Whether teachers and students in Colleges and universities, research institutes, or researchers in clinical scientific research departments of medical institutions, mature commercialized kits can be used to accelerate scientific research progress and improve the reliability and reproducibility of test results. In the future, with the continuous in-depth research on the occurrence, development, prevention and treatment of diseases, such kits may also expand to the clinical detection dimension and open a new world in the field of IVD detection.

  安捷伦液相色谱系统的可靠性和稳定性极佳,系统耐压上限1300 bar,动态流速范围可高达 5
mL/min,适合快速精确分离多种代谢物组分。
Triple quadrupole mass spectrometry has excellent sensitivity, precision and scanning speed, which can accurately quantify even a small amount of samples. These superior performances are the hardware basis for bleck to develop rapid quantitative detection methods. After a large number of different types of samples were tested and the detection methods were improved, commercial kit products were further derived.

Starting from application and meeting the actual needs of scientific researchers is the consistent goal of BlackRock. The targets covered by the kit have been carefully selected. In addition to 20 protein amino acids with rich functions, star molecular neurotransmitters and catecholamines in recent years have also been selected. The content of these two kinds of metabolites in organisms is very low, and it is very difficult to detect them. According to research, they may be related to a variety of diseases. Neurotransmitters have a wide range of regulatory effects on cardiovascular, neurological, endocrine and other organizational systems, and have an important impact on physiological activities such as sleep arousal, emotion, emotion, stress behavior and so on.

  例如上文提到的,在抑郁症相关的研究中,有研究者发现,在抑郁症患者的血浆中GABA通路(神经递质类)和儿茶酚胺通路相关代谢物的浓度与健康人有显著性区别,可能是潜在的诊断抑郁症的标志物。嗜铬细胞瘤和副神经节瘤(PPGL)会合成、分泌和释放大量儿茶酚胺,引起患者血压升高和代谢性改变等一系列临床症候群,并造成心、脑、肾、血管等严重并发症甚至死亡。
“嗜铬细胞瘤和副神经节瘤诊断治疗专家共识(2020版)”中推荐使用LC-MS/MS检测血、尿中的儿茶酚胺用于辅助诊断。

In addition to the diversity of applications, the newly developed kit products of bleck also have the following technical advantages:

 · tandem mass spectrometry:gold standard for quantitative detection of small molecules, with strong specificity, and simultaneous detection of multiple targets at one time

· sensitizing probe:authorized patent (Patent No.:ZL 201610424377.9, patent type:invention patent), specifically binds and carries its own charge, and the sensitivity is improved by at least two orders of magnitude

· small sample quantity:only 20~150 μ L blood sample or 20 μ L urine sample, still excellent for trace substances

· protect sulfhydryl:it has special protection for -sh that is easy to be oxidized, and can accurately detect the correct concentration of cysteine

Functions and applications of the kit

  博莱克科技(武汉)有限公司(简称博莱克)创立于2015年,专注于Metabonomics在科研服务领域的技术开发与应用。在正式推出Metabonomics试剂盒之前,博莱克为北京阜外医院等多家科研机构提供了能覆盖数万种代谢物的检测服务,总结发现了众多科研客户对于部分重点检测目标的高频需求,并基于此着手开发通用试剂盒。

  在2021年安捷伦与博莱克达成战略合作后,双方一直共同致力于液相质谱适配检测试剂盒技术合作开发,推动检测技术在Metabonomics方面的实地应用。在此次试剂盒研发过程中,安捷伦不仅提供了先进、稳定的检测技术platform,而且相关产品专家不断跟进和交流,为产品研发提供了重要指导和帮助。

Bleck technology team

  Metabonomics应用前景广阔,潜力可期,为科学家未来进一步解析复杂生命系统的机理与奥秘指明新的方向、提供有价值的线索,有望引领新一轮健康科技与生物产业变革。而Metabonomics超灵敏、超高通量的测量技术需求必将挑战当今最优秀仪器的性能极限,对仪器提出全新要求并倒逼仪器硬件能力的提升,进而使仪器技术与分析方法再出现“质的飞跃”。

Therefore,In the future, both parties will continue to develop and transform richer quantitative metabolite detection kits to meet the actual needs of scientific research users, and look forward to further application and development of products in the health and medical fields.同时共同发力Metabonomics成果转化领域,探索新靶标、新产品、新应用,打造更多新型的科研乃至临检试剂盒,服务于“中国人健康”。

  安捷伦也将继续与行业合作伙伴通力协作,通过提供安捷伦尖端、稳定、高性能的产品platform,以及专业的服务和支持,助力更多本土企业实现创新和发展。接下来,通过“Liquid hand series”,我们也将分享更多基于安捷伦液相质谱platform实现创新的案例和故事,敬请期待!

  附录:从1999年诞生以来,Metabonomics这门学科经历了20多年快速发展阶段,从基础研究到应用均取得了诸多成果,在中国的发展更是有两个标志性的事件:一是2017年启动的“国际人类表型组计划”,该项目由复旦大学联合中科院上海生命科学研究院、上海交通大学、上海市计量测试技术研究院等共同承担,是上海首批市级科技重大专项之一,由中国科学家发起,细致描绘代表人群的全表型谱,系统解析表型组与基因组的关联,发现人类健康和疾病等表型特征形成的内在规律和生物标志物,Metabonomics是其中重要一环。二是2018年中国生物物理学会Metabonomics分会的成立,标志着我国Metabonomics领域研究人员进入集体共发展的阶段,重点关注行业的人才培养、研究水平提高、规范化、标准化等问题,通过定期举办学术会议、讲习培训班、陆续推出行业标准等一系列举措,促进我国Metabonomics领域的进一步深入发展。